• Product NameMRPS25 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MRPS25 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MRPS25.
• Target NameMRPS25
• Alternative Names    mitochondrial 28S ribosomal protein S25; mitochondrial ribosomal protein S25; MRP-S25; RPMS25; RT25
• Accession No. Swiss-Prot: P82663
  NCBI Gene ID: 64432
• UniprotP82663
• Gene ID64432;
• SDS-PAGE MW 20kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.

Ota T., Nat. Genet. 36-40-45(2004).
Hattori A., DNA Res. 7:357-366(2000).
Koc E.C., J. Biol. Chem. 275:32585-32591(2000).

• Western blot analysis of extracts from 293 cells, using MRPS25 antibody #34792.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting