• Product NameNDUFS5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NDUFS5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human NDUFS5.
• Target NameNDUFS5
• Alternative Names    NADH dehydrogenase [ubiquinone] iron-sulfur protein 5; NADH-ubiquinone oxidoreductase 15 kDa subunit; Complex I-15 kDa; CI-15 kDa;
• Accession No. Swiss-Prot: O43920
  NCBI Gene ID: 4725
• UniprotO43920
• Gene ID4725;
• SDS-PAGE MW 15kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Mao M., Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
Loeffen J., J. Inherit. Metab. Dis. 22:19-28(1999).

• Western blot analysis of extracts from K562 cells, using NDUFS5 antiobdy #34835.
• Immunohistochemistry analysis of paraffin-embedded human heart tissue using NDUFS5 antibody #34835.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting