• Product NameMT-ND1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MT-ND1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MT-ND1.
• Target NameMT-ND1
• Alternative Names    NADH-ubiquinone oxidoreductase chain 1; EC 1.6.5.3; NADH dehydrogenase subunit 1; MT-ND1; MTND1
• Accession No. Swiss-Prot: P03886
  NCBI Gene ID: 4535
• UniprotP03886
• Gene ID4535;
• SDS-PAGE MW 36kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. HAMAP-Rule MF_01350

Anderson S., Nature 290:457-465(1981).
Horai S., Proc. Natl. Acad. Sci. U.S.A. 92:532-536(1995).
Moilanen J.S., Mol. Biol. Evol. 20:2132-2142(2003).

• Western blot analysis of extracts from A549 cells and COLO cells, using MT-ND1 antibody #34839.
• Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using MT-ND1 antibody #34839.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting