• Product NamePTGR1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PTGR1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human PTGR1.
• Target NamePTGR1
• Alternative Names    Prostaglandin reductase 1; PRG-1; NADP-dependent leukotriene B4 12-hydroxydehydrogenase; EC=1.3.1.74; 15-oxoprostaglandin 13-reductase
• Accession No. Swiss-Prot: Q14914
  NCBI Gene ID: 22949
• UniprotQ14914
• Gene ID22949;
• SDS-PAGE MW 36kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity towards PGE1, PGE2 and PGE2-alpha By similarity. Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4.

Humphray S.J., Nature 429:369-374(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Yokomizo T., J. Biol. Chem. 271:2844-2850(1996)

• Western blot analysis of extracts from HT-29 cells, using PTGR1 antibody #34841.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting