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GATA1(Phospho-Ser310) Antibody

11042
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  Catalog No. Package Size Price
11042-1 50ul $189.00
11042-2 100ul $279.00

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Description
  • Product Name GATA1(Phospho-Ser310) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of GATA1 only when phosphorylated at serine 310.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 310 (K-A-S(p)-G-K) derived from Human GATA1.
  • Target Name GATA1
  • Modification Phospho
  • Alternative Names GAT1; GATA1; GF-1; NF-E1;
  • Accession No. Swiss-Prot: P15976
    NCBI Protein: NP_002040.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images
  • GATA1(Phospho-Ser310) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using GATA1(Phospho-Ser310) Antibody #11042.

  • GATA1(Phospho-Ser310) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed K562 cells using GATA1 (Phospho-Ser310) Antibody #11042.

Background

GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.

Kadri Z, et al.(2005)Mol Cell Biol 2005 Sep; 25(17): 7412-22.

    Please let us know if you have published research using #11042 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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