• Product NameNUMA1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NUMA1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human NUMA1.
• Target NameNUMA1
• Alternative Names    Nuclear mitotic apparatus protein 1; NuMA protein; SP-H antigen;
• Accession No. Swiss-Prot: Q14980
  NCBI Gene ID: 4926
• UniprotQ14980
• Gene ID4926;
• SDS-PAGE MW 240kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume. Required for maintenance and establishment of the mitotic spindle poles, functionning as a tether linking bulk microtubules of the spindle to centrosomes. May be involved in coordination of the alignment of the mitotic spindle to the cellular polarity axis, which is a prerequisite for asymmetric cell divisions.

Yang C.H., J. Cell Biol. 116:1303-1317(1992).
Compton D.A., J. Cell Biol. 116:1395-1408(1992).
Maekawa T., J. Cell Sci. 105:589-600(1993).

• Western blot analysis of extracts from A549 cells, using NUMA1 antibody #34869.
• Immunohistochemistry analysis of paraffin-embedded human testis tissue using NUMA1 antibody #34869.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting