• Product NameNOLC1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total NOLC1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human NOLC1.
• Target NameNOLC1
• Alternative Names    140 kDa nucleolar phosphoprotein; HCV NS5A trans-regulated protein 13; HCV NS5A-transactivated protein 13; Hepatitis C virus NS5A-transactivated protein 13; KIAA0035
• Accession No. Swiss-Prot: Q14978
  NCBI Gene ID: 9221
• UniprotQ14978
• Gene ID9221;
• SDS-PAGE MW 74kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.

Pai C.-Y., J. Cell Sci. 108:1911-1920(1995).
Ying Z.L., Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
Deloukas P., Nature 429:375-381(2004).

• Western blot analysis of extracts from K562 cells, using NOLC1 antibody #34876.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting