• Product NamePOLDIP3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total POLDIP3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human POLDIP3.
• Target NamePOLDIP3
• Alternative Names    46 kDa DNA polymerase delta interaction protein; KIAA1649; p46; PDIP3; PDIP46
• Accession No. Swiss-Prot: Q9BY77
  NCBI Gene ID: 84271
• UniprotQ9BY77
• Gene ID84271;
• SDS-PAGE MW 46kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Involved in nuclear mRNA export; probably mediated by assoociation with the TREX complex.

Liu L., J. Biol. Chem. 278:10041-10047(2003).
Hirosawa M., DNA Res. 8:1-9(2001).
Collins J.E., Genome Res. 13:27-36(2003).

• Western blot analysis of extracts from RAW264.7 cells, using POLDIP3 antibody #34909.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting