• Product NameSF3B14 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total SF3B14 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human SF3B14.
• Target NameSF3B14
• Alternative Names    CGI-110; HSPC175; Ht006; PM14; pre-mRNA branch site protein p14
• Accession No. Swiss-Prot: Q9Y3B4
  NCBI Gene ID: 51639
• UniprotQ9Y3B4
• Gene ID51639;
• SDS-PAGE MW 20kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.

Will C.L., EMBO J. 20:4536-4546(2001).
Lai C.-H., Genome Res. 10:703-713(2000).
Zhang Q.-H., Genome Res. 10:1546-1560(2000).

• Western blot analysis of extracts from HepG2 cells, using SF3B14 antibody #34922.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting