• Product NamePDHA1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PDHA1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human PDHA1.
• Target NamePDHA1
• Alternative Names    Pyruvate dehydrogenase E1 component subunit alpha; somatic form; mitochondrial; PDHE1-A type I; PDHA1
• Accession No. Swiss-Prot: P08559
  NCBI Gene ID: 5160
• UniprotP08559
• Gene ID5160;
• SDS-PAGE MW 43kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.

Song B.J., Submitted (APR-1990).
Bentley D.R., Nature 434:325-337(2005).
Comb M.J., Cell 131:1190-1203(2007).

• Western blot analysis of extracts from A549, HepG2 and Jurkat cells, using PDHA1 antibody #34946.
• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PDHA1 antibody #34946.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting