• Product NameRAPGEF5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total RAPGEF5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human RAPGEF5.
• Target NameRAPGEF5
• Alternative Names    GFR; guanine nucleotide exchange factor for Rap1; M-Ras-regulated GEF; M-Ras-regulated Rap GEF; MR-GEF
• Accession No. Swiss-Prot: Q92565
  NCBI Gene ID: 9771
• UniprotQ92565
• Gene ID9771;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation.

Nagase T., DNA Res. 3:321-329(1996).
Scherer S.W., Science 300:767-772(2003).
Ichiba T., FEBS Lett. 457:85-89(1999)

• Western blot analysis of extracts from RAW264.7 cells, using RAPGEF5 antibody #34959.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting