• Product NameRRAGA/B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total RRAGA/B protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human RRAGA/B.
• Target NameRRAGA/B
• Alternative Names    Ras-related GTP-binding protein A; Rag A; RagA; Adenovirus E3 14.7 kDa-interacting protein 1; FIP-1
• Accession No. Swiss-Prot: Q7L523/Q5VZM2
  NCBI Gene ID: 10670/10325
• UniprotQ7L523
• Gene ID10670;
• SDS-PAGE MW 34kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.

Schuermann A., J. Biol. Chem. 270:28982-28988(1995).
Li Y., J. Virol. 71:1576-1582(1997).
Hirose E., J. Cell Sci. 111:11-21(1998).

• Western blot analysis of extracts from HepG2 cells, using RRAGA/B antibody #34965.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting