• Product NameRGS10 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RGS10 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human RGS10.
• Target NameRGS10
• Alternative Names    Regulator of G-protein signaling 10; RGS10;
• Accession No. Swiss-Prot: O43665
  NCBI Gene ID: 6001
• UniprotO43665
• Gene ID6001;
• SDS-PAGE MW 20kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Associates specifically with the activated forms of the G protein subunits G(i)-alpha and G(z)-alpha but fails to interact with the structurally and functionally distinct G(s)-alpha subunit. Activity on G(z)-alpha is inhibited by palmitoylation of the G-protein.

Hunt T.W., Nature 383:175-177(1996).
Chatterjee T.K., J. Biol. Chem. 275:24013-24021(2000).
Burgon P.G., J. Biol. Chem. 276:32828-32834(2001).

• Western blot analysis of extracts from Jurkat cells, using RGS10 antibody #34980.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting