• Product NameARHGEF3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ARHGEF3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ARHGEF3.
• Target NameARHGEF3
• Alternative Names    ARHG3; DKFZP434F2429; exchange factor found in platelets and leukemic and neuronal tissues; GEF3; MGC118905
• Accession No. Swiss-Prot: Q9NR81
  NCBI Gene ID: 50650
• UniprotQ9NR81
• Gene ID50650;
• SDS-PAGE MW 55kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.

Thiesen S., Biochem. Biophys. Res. Commun. 273:364-369(2000).
Wiemann S., Genome Res. 11:422-435(2001).
Guo J.H., Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.

• Western blot analysis of extracts from COLO cells, using ARHGEF3 antibody #34995.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting