• Product NameRUFY1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RUFY1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human RUFY1.
• Target NameRUFY1
• Alternative Names    FYVE-finger protein EIP1; Zinc finger FYVE domain-containing protein 12; La-binding protein 1; Rab4-interacting protein;
• Accession No. Swiss-Prot: Q96T51
  NCBI Gene ID: 80230
• UniprotQ96T51
• Gene ID80230;
• SDS-PAGE MW 80kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.

Fouraux M.A., Mol. Biol. Cell 15:611-624(2004).
Ota T., Nat. Genet. 36:40-45(2004).
Yang J., J. Biol. Chem. 277:30219-30226(2002).

• Western blot analysis of extracts from HeLa cells, using RUFY1 antibody #35009.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting