• Product NamePPP2R5A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PPP2R5A protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human PPP2R5A.
• Target NamePPP2R5A
• Alternative Names    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform; PP2A B subunit isoform B'-alpha; PP2A B subunit isoform B56-alpha; PP2A B subunit isoform PR61-alpha; PR61alpha
• Accession No. Swiss-Prot: Q15172
  NCBI Gene ID: 5525
• UniprotQ15172
• Gene ID5525;
• SDS-PAGE MW 57kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

McCright B., J. Biol. Chem. 270:26123-26128(1995).
Ota T., Nat. Genet. 36:40-45(2004).
Gregory S.G., Nature 441:315-321(2006).

• Western blot analysis of extracts from HuvEc cells and HeLa cells, using PPP2R5A antibody #35029.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting