• Product NameCD1E Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CD1E protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CD1E.
• Target NameCD1E
• Alternative Names    T-cell surface glycoprotein CD1e; CD1e antigen; R2G1; CD1E;
• Accession No. Swiss-Prot: P15812
  NCBI Gene ID: 913
• UniprotP15812
• Gene ID913;
• SDS-PAGE MW 36kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

T-cell surface glycoprotein CD1e, soluble binds diacetylated lipids, including phosphatidyl inositides and diacylated sulfoglycolipids, and is required for the presentation of glycolipid antigens on the cell surface. The membrane-associated form is not active.

Calabi F., Eur. J. Immunol. 19:285-292(1989).
Angenieux C., J. Biol. Chem. 275:37757-37764(2000).
Gregory S.G., Nature 441:315-321(2006).

• Western blot analysis of extracts from HuvEc cells and HepG2 cells, using CD1E antibody #35089.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting