• Product NameUBE2T Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total UBE2T protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human UBE2T.
• Target NameUBE2T
• Alternative Names    Ubiquitin-conjugating enzyme E2 T; Ubiquitin-protein ligase T; Ubiquitin carrier protein T; UBE2T;
• Accession No. Swiss-Prot: Q9NPD8
  NCBI Gene ID: 29089
• UniprotQ9NPD8
• Gene ID29089;
• SDS-PAGE MW 22kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.

Okaze H., Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Zhang Q.-H., Genome Res. 10:1546-1560(2000).

• Western blot analysis of extracts from HeLa cells, Jurkat cells and K562 cells, using UBE2T antibody #35133.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting