• Product NameWASF2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total WASF2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human WASF2.
• Target NameWASF2
• Alternative Names    Wiskott-Aldrich syndrome protein family member 2WASP family protein member 2; Protein WAVE-2; Verprolin homology domain-containing protein 2; WASF2;
• Accession No. Swiss-Prot: Q9Y6W5
  NCBI Gene ID: 10163
• UniprotQ9Y6W5
• Gene ID10163;
• SDS-PAGE MW 54kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.

Suetsugu S., Biochem. Biophys. Res. Commun. 260:296-302(1999).
Gregory S.G., Nature 441:315-321(2006).
Miki H., Nature 408:732-735(2000).

• Western blot analysis of extracts from 293 cells, using WASF2 antibody #35147.
• Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using WASF2antibody #35147.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting