• Product NameZC3H8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ZC3H8 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ZC3H8.
• Target NameZC3H8
• Alternative Names    Zinc finger CCCH domain-containing protein 8; ZC3H8; ZC3HDC8;
• Accession No. Swiss-Prot: Q8N5P1
  NCBI Gene ID: 84524
• UniprotQ8N5P1
• Gene ID84524;
• SDS-PAGE MW 40kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene By similarity. Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

Zhuang D.Z., Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Hwang E.S., Immunology 106:464-469(2002).

• Western blot analysis of extracts from HepG2 cells, using ZC3H8 antibody #35154.
• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ZC3H8 antibody #35154.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting