• Product NameZP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ZP1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ZP1.
• Target NameZP1
• Alternative Names    mgc87693; zona pellucida glycoprotein 1; zona pellucida glycoprotein 1 (sperm receptor); zp1; zp1 protein
• Accession No. Swiss-Prot: P60852
  NCBI Gene ID: 22917
• UniprotP60852
• Gene ID22917;
• SDS-PAGE MW 70kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.

Hughes D.C., Biochim. Biophys. Acta 1447:303-306(1999).
Taylor T.D., Nature 440:497-500(2006).

• Western blot analysis of extracts from LOVO cells, using ZP1 antibody #35166.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting