• Product NameMED27 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MED27 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human MED27.
• Target NameMED27
• Alternative Names    Cofactor required for Sp1 transcriptional activation subunit 8; cofactor required for Sp1 transcriptional activation; subunit 8 (34kD); cofactor required for Sp1 transcriptional activation; subunit 8
• Accession No. Swiss-Prot: Q6P2C8
  NCBI Gene ID: 9442
• UniprotQ6P2C8
• Gene ID9442;
• SDS-PAGE MW 35kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Humphray S.J., Nature 429:369-374(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Ryu S., Nature 397:446-450(1999).

• Western blot analysis of extracts from HeLa cells, using MED27 antibody #35183.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting