• Product NameSIRT3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SIRT3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human SIRT3.
• Target NameSIRT3
• Alternative Names    NAD-dependent deacetylase sirtuin-3; mitochondrial; SIR2-like protein 3; hSIRT3; SIRT3
• Accession No. Swiss-Prot: Q9NTG7
  NCBI Gene ID: 23410
• UniprotQ9NTG7
• Gene ID23410;
• SDS-PAGE MW 45kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.

Bechtel S., BMC Genomics 8:399-399(2007).
Schwer B., J. Cell Biol. 158:647-657(2002).
Onyango P., Proc. Natl. Acad. Sci. U.S.A. 99:13653-13658(2002).

• Western blot analysis of extracts from COLO205 cells, using SIRT3 antibody #35202.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting