• Product NameIFIT5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total IFIT5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human IFIT5.
• Target NameIFIT5
• Alternative Names    IFIT-5; interferon-induced protein with tetratricopeptide repeats 5; retinoic acid- and interferon-inducible 58 kDa protein; RI58;
• Accession No. Swiss-Prot: Q13325
  NCBI Gene ID: 24138
• UniprotQ13325
• Gene ID24138;
• SDS-PAGE MW 50kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Also recognizes and binds tRNAs.

Rogers J., Nature 429:375-381(2004).
Venter J.C., Submitted (SEP-2005).
Bennett K.L., Submitted (OCT-2008).

• Western blot analysis of extracts from Jurkat cells, using IFIT5 antibody #35225.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting