• Product NameNRF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total NRF1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human NRF1.
• Target NameNRF1
• Alternative Names    Nuclear respiratory factor 1; NRF-1; Alpha palindromic-binding protein; Alpha-pal; NRF1
• Accession No. Swiss-Prot: Q16656
  NCBI Gene ID: 4899
• UniprotQ16656
• Gene ID4899;
• SDS-PAGE MW 53kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Virbasius C.A., Genes Dev. 7:2431-2445(1993).
Efiok B.J.S., J. Biol. Chem. 269:18921-18930(1994).
Gopalakrishnan L., J. Biol. Chem. 270:18019-18025(1995).

• Western blot analysis of extracts from 3T3 cells, using NRF1 antibody #35250.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting