• Product NameBAG3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total BAG3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human BAG3.
• Target NameBAG3
• Alternative Names    BAG-3; BAG-family molecular chaperone regulator-3; BCL-2 binding athanogene- 3; Bcl-2-binding protein Bis; BIS
• Accession No. Swiss-Prot: O95817
  NCBI Gene ID: 9531
• UniprotO95817
• Gene ID9531;
• SDS-PAGE MW 62kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.

Takayama S., J. Biol. Chem. 274:781-786(1999).
Lee J.H., Oncogene 18:6183-6190(1999).
Doong H., Oncogene 19:4385-4395(2000).

• Western blot analysis of extracts from 293 cells, using BAG3 antibody #35276.
• Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using BAG3 antibody #35276.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting