• Product NameMRCKa Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MRCKa protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human MRCKa.
• Target NameMRCKa
• Alternative Names    CDC42 binding protein kinase alpha (DMPK-like); CDC42 binidng protein kinase beta; CDC42-binding protein kinase alpha; CDC42-binding protein kinase alpha (DMPK-like); CDC42BPA
• Accession No. Swiss-Prot: Q5VT25
  NCBI Gene ID: 8476
• UniprotQ5VT25
• Gene ID8476;
• SDS-PAGE MW 197kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake.

Wilkinson S., Nat. Cell Biol. 7:255-261(2005).
Totoki Y., Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Bechtel S., BMC Genomics 8:399-399(2007).

• Western blot analysis of extracts from Jurkat/COLO205 cells, using MRCKa antibody #35291.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting