• Product NamePHKG1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PHKG1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human PHKG1.
• Target NamePHKG1
• Alternative Names    KPBG; PHKG; Phosphorylase B kinase gamma catalytic chain skeletal muscle isoform; Phosphorylase kinase gamma subunit 1;
• Accession No. Swiss-Prot: Q16816
  NCBI Gene ID: 5260
• UniprotQ16816
• Gene ID5260;
• SDS-PAGE MW 45kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 By similarity.

Wehner M., Hum. Genet. 96:616-618(1995).
Burwinkel B. Jr., Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
Greenman C., Nature 446:153-158(2007).

• Western blot analysis of extracts from HuvEc cells and HepG2 cells, using PHKG1 antibody #35293.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting