• Product NameTLK1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total TLK1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human TLK1.
• Target NameTLK1
• Alternative Names    kinase TLK1; PKU-beta; tousled-like kinase 1;
• Accession No. Swiss-Prot: Q9UKI8
  NCBI Gene ID: 9874
• UniprotQ9UKI8
• Gene ID9874;
• SDS-PAGE MW 87kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.

Yamakawa A., Gene 202:193-201(1997).
Sillje H.H.W., EMBO J. 18:5691-5702(1999).
Cabaniols J.-P., Mol. Biol. Cell 10:4033-4041(1999).

• Western blot analysis of extracts from rat brain cells and NIH/3T3 cells, using TLK1 antibody #35297.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting