• Product NameTOLLIP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total TOLLIPprotein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human TOLLIP.
• Target NameTOLLIP
• Alternative Names    adapter protein; FLJ33531; IL-1RAcPIP; TOLIP; toll interacting protein
• Accession No. Swiss-Prot: Q9H0E2
  NCBI Gene ID: 54472
• UniprotQ9H0E2
• Gene ID54472;
• SDS-PAGE MW 30kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.

Burns K., Nat. Cell Biol. 2:346-351(2000).
Wiemann S., Genome Res. 11:422-435(2001).
Zhang G., J. Biol. Chem. 277:7059-7065(2002).

• Western blot analysis of extracts from HuvEc/Jurkat/293 cells, using TOLLIP antibody #35328.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting