• Product NameGFAP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu Rt Ms
• SpecificityThe antibody detects endogenous levels of total GFAP protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 190 and 432 of GFAP.
• Target NameGFAP
• Alternative Names    FLJ45472 antibody; GFAP antibody; glial fibrillary acidic protein antibody
• Accession No. Swiss-Prot#:P14136;
  NCBI Gene#:2670
• UniprotP14136
• Gene ID2670;
• SDS-PAGE MW 50kd
• Concentration 1.01mg/ml
• Formulation Rabbit IgG in 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:3000
  

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

• GFAP antibody detects GFAP protein by western blot analysis.
  A. 30 μg U87-MG whole cell extract
  B. 30 μg IMR32 whole cell extract
  C. 50 μg mouse brain extract
  D. 50 μg rat brain extract
  10 % SDS-PAGE
  #35350 diluted at 1:2500
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting