• Product NameOct4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total Oct4 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 101 and 325 of OCT3/4.
• Target NameOct4
• Alternative Names    MGC22487 antibody; OTF-3 antibody; OTF3 antibody; OTF4 antibody; POU5F1 antibody; POU-type homeodomain-containing DNA-binding protein antibody; octamer-binding protein 3 antibody; POU domain transcription factor OCT4 antibody; octamer-binding protein 4 an
• Accession No. Swiss-Prot#:Q01860;
  NCBI Gene#:5460
• UniprotQ01860
• Gene ID5460;
• SDS-PAGE MW 39kd
• Concentration 0.48mg/ml
• Formulation Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:3000

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

• Sample (20 ug of whole cell lysate)
  A: human ESC
  10% SDS PAGE
  #35351 diluted at 1:500
  
• Sample (30 ug of whole cell lysate)
  
  A: NIH-3T3
  
  10% SDS PAGE
  
  OCT3/4 antibody
  
  #35351 diluted at 1:1000
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting