• Product NameProthrombin Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Prothrombin protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment contain a sequence corresponding to a region within amino acids 9 and 226 of Prothrombin.
• Target NameProthrombin
• Alternative Names    PT antibody; F2 antibody; prothrombin antibody; serine protease antibody; prothrombin B-chain antibody; coagulation factor II (thrombin) antibody
• Accession No. Swiss-Prot#:P00734;
  NCBI Gene#:2147
• UniprotP00734
• Gene ID2147;
• SDS-PAGE MW 70kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:3000
  

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: 293T
  B: A431
  7.5% SDS PAGE
  #35360 diluted at 1:1000
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting