• Product NameBLM Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsIF WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total BLM protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 30 and 385 of BLM.
• Target NameBLM
• Alternative Names    BS antibody; MGC126616 antibody; MGC131618 antibody; MGC131620 antibody; RECQ2 antibody; RECQL2 antibody; RECQL3 antibody; BLM antibody; "DNA helicase; RecQ-like type 2 antibody"; Bloom syndrome protein antibody; recQ protein-like 3 antibody; "Bloom syndr
• Accession No. Swiss-Prot#:P54132;
  NCBI Gene#:641
• UniprotP54132
• Gene ID641;
• SDS-PAGE MW 159kd
• Concentration 0.93mg/ml
• Formulation Rabbit IgG in 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:1000

The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq]

• BLM antibody detects BLM protein by western blot analysis.
  A. 30 μg Jurkat whole cell lysate/extract
  B. 30 μg Raji whole cell lysate/extract
  C. 30 μg NCI-H929 whole cell lysate/extract
  5 % SDS-PAGE
  #35370 diluted at 1:500
  
• BLM antibody, N-term detects BLM protein at nucleus by immunofluorescent analysis.
  Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
  Green: BLM protein stained by BLM antibody, N-term (#35370) diluted at 1:500.
  Blue: Hoechst 33342 staining.
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting