• Product NameEIF4A2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total EIF4A2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment contain a sequence corresponding to a region within amino acids 1 and 96 of EIF4A2.
• Target NameEIF4A2
• Alternative Names    BM-010 antibody; DDX2B antibody; EIF4A antibody; EIF4F antibody; eIF-4A-II antibody; eIF4A-II antibody; EIF4A2 antibody; "eukaryotic translation initiation factor 4A; isoform 2 antibody"; ATP-dependent RNA helicase eIF4A-2 antibody; eukaryotic initiation
• Accession No. Swiss-Prot#:Q14240;
  NCBI Gene#:1974
• UniprotQ14240
• Gene ID1974;
• SDS-PAGE MW 46kd
• Concentration 0.23mg/ml
• Formulation Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:100-1:1000
  

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

• Sample(30 μg of whole cell lysate)
  A:293T
  B:A431
  12% SDS PAGE
  #35372 diluted at 1:200

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting