• Product NameTyrosine Hydroxylase Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsIF WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Tyrosine Hydroxylase protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 1 and 270 of Tyrosine Hydroxylase.
• Target NameTyrosine Hydroxylase
• Alternative Names    DYT14 antibody; DYT5b antibody; TYH antibody; TH antibody; dystonia 14 antibody; tyrosine 3-hydroxylase antibody; tyrosine 3-monooxygenase antibody; tyrosine hydroxylase antibody
• Accession No. Swiss-Prot#:P07101;
  NCBI Gene#:7054
• UniprotP07101
• Gene ID7054;
• SDS-PAGE MW 59kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:1000

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: IMR32
  7.5% SDS PAGE
  #35392 diluted at 1:1000
  
• Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.
  Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
  Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody (#35392) diluted at 1:500.
  Blue: Hoechst 33342 staining.
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting