• Product NameGrp78 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total Grp78 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 478 and 654 of Grp78.
• Target NameGrp78
• Alternative Names    BIP antibody; FLJ26106 antibody; GRP78 antibody; MIF2 antibody; HSPA5 antibody; immunoglobulin heavy chain-binding protein antibody; 78 kDa glucose-regulated protein antibody; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody; "heat shoc
• Accession No. Swiss-Prot#:P11021;
  NCBI Gene#:3309
• UniprotP11021
• Gene ID3309;
• SDS-PAGE MW 72kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:3000
  

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

• Sample (30 ug of whole cell lysate)
  A: NIH-3T3
  B: JC
  C: BCL-1
  7.5% SDS PAGE
  #35394 diluted at 1:2000
  
• Grp78 antibody detects Grp78 protein by western blot analysis.
  A. 30 μg 293T whole cell lysate/extract
  B. 30 μg A431 whole cell lysate/extract
  C. 30 μg HeLa whole cell lysate/extract
  D. 30 μg HepG2 whole cell lysate/extract
  7.5 % SDS-PAGE
  #102567 dilution: 1:1000

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting