• Product NamePyruvate Kinase (liver/RBC) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu Rt Ms
• SpecificityThe antibody detects endogenous levels of total Pyruvate Kinase (liver/RBC) protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 1 and 301 of Pyruvate Kinase .
• Target NamePyruvate Kinase (liver/RBC)
• Alternative Names    PK1 antibody; PKL antibody; PKR antibody; PKRL antibody; RPK antibody; PKLR antibody; R-type/L-type pyruvate kinase antibody; pyruvate kinase type L antibody; red cell/liver pyruvate kinase antibody; pyruvate kinase 1 antibody; pyruvate kinase isozyme R/L
• Accession No. Swiss-Prot#:P30613;
  NCBI Gene#:5313
• UniprotP30613
• Gene ID5313;
• SDS-PAGE MW 62kd
• Concentration 0.62mg/ml
• Formulation Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:20000
  

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

• Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
  A. 30 ?g K562 whole cell lysate/extract
  7.5 % SDS-PAGE
  #35442 diluted at 1:1000
  
• Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
  A. 30 ?g Raw264.7 whole cell lysate/extract
  7.5 % SDS-PAGE
  #35442 dilution: 1:1000
• Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
  A. 50 ?g rat liver lysate/extract
  7.5 % SDS-PAGE
  #35442 dilution: 1:500
• Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein by western blot analysis.
  A. 30 μg 293T whole cell lysate/extract
  B. 5 μg whole cell lysate/extract of human PKLR-transfected 293T cells
  7.5 % SDS-PAGE
  #35442 diluted at 1:10000

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting