• Product NameNUMB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NUMB protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 1 and 244 of NUMB.
• Target NameNUMB
• Alternative Names    S171 antibody; NUMB antibody; protein numb homolog antibody; h-Numb antibody; numb homolog (Drosophila) antibody
• Accession No. Swiss-Prot#:P49757;
  NCBI Gene#:8650
• UniprotP49757
• Gene ID8650;
• SDS-PAGE MW 71kd
• Concentration 0.79mg/ml
• Formulation Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:3000
  

The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: A431
  7.5% SDS PAGE
  #35497 diluted at 1:1000
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting