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FLAG tag Antibody

35535
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  Catalog No. Package Size Price
35535 100ul $279.00

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Description
  • Product Name FLAG tag Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification.
  • Applications WB Elisa
  • Specificity The antibody detects transfected proteins contanining FLAG tag.
  • Immunogen Type Peptide
  • Immunogen Description Synthetic peptide: DYKDDDDK conjugated to KLH
  • Target Name FLAG tag
  • Alternative Names DDDDK epitope tag; DYKDDDDK epitope tag
  • SDS-PAGE MW 54kd
  • Concentration 1.2mg/ml
  • Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:5000-1:20000

ELISA dilution: 1:5000-1:10000

Images
  • FLAG tag Antibody - SAB | Signalway Antibody

    Gel: 8%SDS-PAGELysate: 0.1/ 0.2/ 0.5 μgLane 1-3: Fusion proteins containing the flag TagPrimary antibody: 35535(FLAG Tag Antibody) at dilution 1/ 20000Secondary antibody: Goat anti rabbit IgG at 1/5000 dilutionExposure time: 1 second

Background

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.

  • TaoChen, Jingjie Li et al, et al,PKCε phosphorylates MIIP and promotes colorectal cancer metastasis through inhibition of RelA deacetylation., Nature Communications, 8(1):939. doi: 10.1038/s41467-017-01024-2. (2017 Oct 16),
    PMID: 29038521
  • Ting Wang,Qiujing Yu Li et al et al,GlcNAcylation of fumarase maintains tumour growth under glucose deficiency., Nature Cell Biology, doi: 10.1038/ncb3562 (2017),
    PMID: 28628081
  • Please let us know if you have published research using #35535 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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