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GFP Antibody

35538
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  Catalog No. Package Size Price
35538 100ul $279.00

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Description
  • Product Name GFP Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification.
  • Applications WB
  • Specificity The antibody detects transfected proteins contanining GFP tag.
  • Immunogen Type Recombinant Protein
  • Immunogen Description Full length fusion protein
  • Target Name GFP
  • Alternative Names GFP-like chromoprotein
  • Accession No. Gene Accssion: P42212
  • SDS-PAGE MW 27kd
  • Concentration 1.6mg/ml
  • Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:1000-1:10000

Images
  • GFP Antibody - SAB | Signalway Antibody

    Gel: 8%SDS-PAGE
    Lysate: 20ug GFP transfected 293T cell
    Primary antibody: 1/800 dilution
    Secondary antibody dilution: 1/8000
    Exposure time: 30 seconds

  • GFP Antibody - SAB | Signalway Antibody

    Gel: 8%SDS-PAGE
    Lysates: 20ug GFP transfected 293T cell per lane
    Primary antibody: at various dilution
    Secondary antibody dilution: 1/8000
    Exposure time: 30 seconds

Background

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.

    Please let us know if you have published research using #35538 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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