• Product NameBNIP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total BNIP1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the N terminal of human BCL2/adenovirus E1B 19kDa interacting protein 1
• Target NameBNIP1
• Alternative Names    NIP1; SEC20; TRG-8
• Accession No. Swiss-Prot#: Q12981
  NCBI Gene ID: 662
  Gene Accssion: BC010959
• UniprotQ12981
• Gene ID662;
• SDS-PAGE MW 26kd
• Concentration 2.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. In addition, this protein is involved in vesicle transport into the endoplasmic reticulum. Alternative splicing of this gene results in four protein products with identical N- and C-termini.?

• Gel: 12%SDS-PAGE
  Lysates (from left to right): A431 and A172 cell, human liver cancer tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/550 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #35597 at dilution 1/60.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting