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SPDEF Antibody

35874
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  Catalog No. Package Size Price
35874 100ul $279.00

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Description
  • Product Name SPDEF Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification.
  • Applications ELISA, WB
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous levels of total SPDEF protein.
  • Immunogen Type Recombinant Protein
  • Immunogen Description Fusion protein corresponding to a region derived from internal residues of human SAM pointed domain containing ets transcription factor
  • Target Name SPDEF
  • Alternative Names PDEF; bA375E1.3; RP11-375E1__A.3
  • Accession No. Swiss-Prot#: O95238
    NCBI Gene ID: 25803
    Gene Accssion: BC021299
  • Concentration 0.6mg/ml
  • Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • Storage Store at -20˚C
Application Details

Western blotting:1:1000-5000

Images
  • SPDEF Antibody - SAB | Signalway Antibody

    Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-3: Human cerebella tissue, Human cerebrum tissue, Mouse brain tissue lysates Primary antibody: SPDEF Antibody at dilution 1/600 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 10 seconds

Background

The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

    Please let us know if you have published research using #35874 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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