• Product NameRCHY1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RCHY1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFull length fusion protein
• Target NameRCHY1
• Alternative Names    ARNIP; CHIMP; PIRH2; PIRH2E; PIRH2F; RNF199; ZNF363; hARNIP; PRO1996
• Accession No. Swiss-Prot#: Q96PM5
  NCBI Gene ID: 25898
  Gene Accssion: BC047393
• UniprotQ96PM5
• Gene ID25898;
• SDS-PAGE MW 30kd
• Concentration 0.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including TP53, HDAC1 and CDKN1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

• Gel: 10%SDS-PAGE
  Lysate: 40ug Hela cell
  Primary antibody: 1/300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minutes
• Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #35879 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting