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ACAD11 Antibody

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  Catalog No. Package Size Price
36016 100ul $279.00

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  • Product Name ACAD11 Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification.
  • Applications WB IHC
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous levels of total ACAD11 protein.
  • Immunogen Type Recombinant Protein
  • Immunogen Description Fusion protein corresponding to a region derived from internal residues of human Acyl-CoA dehydrogenase family, member 11
  • Target Name ACAD11
  • Alternative Names ACAD-11
  • Accession No. Swiss-Prot#: Q709F0
    NCBI Gene ID: 84129
    Gene Accssion: BC125204
  • SDS-PAGE MW 87kd
  • Concentration 0.7mg/ml
  • Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:200-1:1000
Immunohistochemistry: 1:15-1:50

  • ACAD11 Antibody - SAB | Signalway Antibody

    Gel: 8%SDS-PAGE
    Lysates (from left to right): Human fetal liver tissue
    Amount of lysate: 40ug per lane
    Primary antibody: 1/300 dilution
    Secondary antibody dilution: 1/8000
    Exposure time: 1 minute

  • ACAD11 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #36016 at dilution 1/15.

The deduced full-length?ACAD11?protein contains an N-terminal aminoglycoside phosphotransferase domain, followed by a mitochondria localization signal and ACAD N-terminal, middle, and C-terminal domains. It also has a glycosylation site and a C-terminal peroxisome-targeting signal. The ACAD domain contains a catalytic aspartate rather than the catalytic glutamate found in most other ACADs. Real-time RT-PCR detected variable?ACAD11?expression in all tissues examined, with highest expression in adult brain, followed by heart, liver, spinal cord, and kidney. Western blot analysis of fractionated human cerebellum and kidney revealed thatACAD11?associated with mitochondrial membranes, but not mitochondrial matrix. Immunohistochemical analysis showed that?ACAD11colocalized with a mitochondrial marker in human neuroblastoma cells.
    Please let us know if you have published research using #36016 so that we can cite your reference.
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