• Product NameAKR1D1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total AKR1D1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFull length fusion protein
• Target NameAKR1D1
• Alternative Names    CBAS2; SRD5B1; 3o5bred
• Accession No. Swiss-Prot#: P51857
  NCBI Gene ID: 6718
  Gene Accssion: BC130625
• UniprotP51857
• Gene ID6718;
• SDS-PAGE MW 37kd
• Concentration 1.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Human liver cancer tissue
  Amount of lysate: 80ug per lane
  Primary antibody: 1/650 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using #36072 at dilution 1/35.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting