• Product NameFAK(Phospho-Tyr861) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of FAK only when phosphorylated at tyrosine 861.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 861 (H-I-Y(p)-Q-P) derived from Human FAK.
• Target NameFAK
• ModificationPhospho
• Alternative Names    FADK 1; FAK1; PTK2
• Accession No. Swiss-Prot: Q05397
  NCBI Protein: NP _005598.3
• UniprotQ05397
• Gene ID5747;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 125kd
  Western blotting: 1:500~1:1000
  

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Shi Q, et al. (2003) Mol Biol Cell; 14(10): 4306-15.
Vadlamudi RK, et al. (2003) FEBS Lett; 543(1-3): 76-80.
Eliceiri BP, et al. (2002) J Cell Biol Apr 01; 157(1): 149-60.
Abu-Ghazaleh R, (2001) et al. Biochem J; 360(Pt 1): 255-64.

• Western blot analysis of extracts from HT29 cells untreated or treated with EGF using FAK(Phospho-Tyr861) Antibody #11059.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting