• Product NameFTO Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total FTO protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFull length fusion protein
• Target NameFTO
• Alternative Names    ALKBH9
• Accession No. Swiss-Prot#: Q9C0B1
  NCBI Gene ID: 79068
  Gene Accssion: BC003583
• UniprotQ9C0B1
• Gene ID79068;
• SDS-PAGE MW 58kd
• Concentration 2.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:100-1:300

This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): Human fetal brain tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/650 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 minutes
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #36489 at dilution 1/60.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting