• Product NameNDUFAF2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NDUFAF2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human NADH dehydrogenase (ubiquinone) complex I, assembly factor 2
• Target NameNDUFAF2
• Alternative Names    MMTN; B17.2L; mimitin; NDUFA12L
• Accession No. Swiss-Prot#: Q8N183
  NCBI Gene ID: 91942
  Gene Accssion: BC033965/Q8N183
• UniprotQ8N183
• Gene ID91942;
• SDS-PAGE MW 20kd
• Concentration 2.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  

NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene cause progressive encephalopathy resulting from mitochondrial complex I deficiency.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Raji cell and human liver cancer tissue, hela cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/250 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting