• Product NameNDUFS3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total NDUFS3 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
• Target NameNDUFS3
• Alternative Names    CI-30
• Accession No. Swiss-Prot#: O75489
  NCBI Gene ID: 4722
  Gene Accssion: BC000617/O75489
• UniprotO75489
• Gene ID4722;
• SDS-PAGE MW 30kd
• Concentration 1.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Mouse brain and human fetal brain tissue, hela, Raw264.7, NIH/3T3 and 293T cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using #36641 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting